bioactive il 12 p70  (R&D Systems)

Journal:

Article Title: Bacterial Probiotic Modulation of Dendritic Cells

doi: 10.1128/IAI.72.6.3299-3309.2004

Figure Lengend Snippet: Probiotic stimulates IL-10 release in DC cultures. BM cells were grown in GM-CSF. Various concentrations of probiotic or bacteria were added on day 2 for a further 3 days. DC culture supernatants were harvested on day 5, and cytokine levels were measured by ELISA. Results are shown for IL-10 (A) and IL-12 (p70) (B). Error bars represent standard deviations from duplicate wells. P values indicate levels of significance above control PBS. Results are representative of three separate experiments.

Article Snippet: Cytokine detection was done by enzyme-linked immunosorbent assay (ELISA) for interleukin-10 (IL-10) and gamma interferon (IFN-γ) with paired antibodies (PharMingen) and for bioactive IL-12 (p70) with the Duoset DY 419 kit (R&D Systems).

Techniques: Bacteria, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Bacterial Probiotic Modulation of Dendritic Cells

doi: 10.1128/IAI.72.6.3299-3309.2004

Figure Lengend Snippet: Probiotic stimulation of IL-12 (p70). Cells at day 4 of BM DC culture were stimulated for an overnight period by the addition of 107 organisms of probiotic or bacteria/ml until the next day. Supernatants were collected, and IL-12 levels (A) or IL-10 release (B) in supernatants were determined by ELISA. Standard deviation is shown for duplicate wells. The level of significance is indicated by the P value.

Article Snippet: Cytokine detection was done by enzyme-linked immunosorbent assay (ELISA) for interleukin-10 (IL-10) and gamma interferon (IFN-γ) with paired antibodies (PharMingen) and for bioactive IL-12 (p70) with the Duoset DY 419 kit (R&D Systems).

Techniques: Bacteria, Enzyme-linked Immunosorbent Assay, Standard Deviation