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bioactive il 12 p70  (R&D Systems)


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    Structured Review

    R&D Systems bioactive il 12 p70
    Probiotic stimulates IL-10 release in DC cultures. BM cells were grown in GM-CSF. Various concentrations of probiotic or bacteria were added on day 2 for a further 3 days. DC culture supernatants were harvested on day 5, and cytokine levels were measured by ELISA. Results are shown for IL-10 (A) and <t>IL-12</t> <t>(p70)</t> (B). Error bars represent standard deviations from duplicate wells. P values indicate levels of significance above control PBS. Results are representative of three separate experiments.
    Bioactive Il 12 P70, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioactive il 12 p70/product/R&D Systems
    Average 94 stars, based on 144 article reviews
    bioactive il 12 p70 - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "Bacterial Probiotic Modulation of Dendritic Cells"

    Article Title: Bacterial Probiotic Modulation of Dendritic Cells

    Journal:

    doi: 10.1128/IAI.72.6.3299-3309.2004

    Probiotic stimulates IL-10 release in DC cultures. BM cells were grown in GM-CSF. Various concentrations of probiotic or bacteria were added on day 2 for a further 3 days. DC culture supernatants were harvested on day 5, and cytokine levels were measured by ELISA. Results are shown for IL-10 (A) and IL-12 (p70) (B). Error bars represent standard deviations from duplicate wells. P values indicate levels of significance above control PBS. Results are representative of three separate experiments.
    Figure Legend Snippet: Probiotic stimulates IL-10 release in DC cultures. BM cells were grown in GM-CSF. Various concentrations of probiotic or bacteria were added on day 2 for a further 3 days. DC culture supernatants were harvested on day 5, and cytokine levels were measured by ELISA. Results are shown for IL-10 (A) and IL-12 (p70) (B). Error bars represent standard deviations from duplicate wells. P values indicate levels of significance above control PBS. Results are representative of three separate experiments.

    Techniques Used: Bacteria, Enzyme-linked Immunosorbent Assay

    Probiotic stimulation of IL-12 (p70). Cells at day 4 of BM DC culture were stimulated for an overnight period by the addition of 107 organisms of probiotic or bacteria/ml until the next day. Supernatants were collected, and IL-12 levels (A) or IL-10 release (B) in supernatants were determined by ELISA. Standard deviation is shown for duplicate wells. The level of significance is indicated by the P value.
    Figure Legend Snippet: Probiotic stimulation of IL-12 (p70). Cells at day 4 of BM DC culture were stimulated for an overnight period by the addition of 107 organisms of probiotic or bacteria/ml until the next day. Supernatants were collected, and IL-12 levels (A) or IL-10 release (B) in supernatants were determined by ELISA. Standard deviation is shown for duplicate wells. The level of significance is indicated by the P value.

    Techniques Used: Bacteria, Enzyme-linked Immunosorbent Assay, Standard Deviation



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    Image Search Results


    Probiotic stimulates IL-10 release in DC cultures. BM cells were grown in GM-CSF. Various concentrations of probiotic or bacteria were added on day 2 for a further 3 days. DC culture supernatants were harvested on day 5, and cytokine levels were measured by ELISA. Results are shown for IL-10 (A) and IL-12 (p70) (B). Error bars represent standard deviations from duplicate wells. P values indicate levels of significance above control PBS. Results are representative of three separate experiments.

    Journal:

    Article Title: Bacterial Probiotic Modulation of Dendritic Cells

    doi: 10.1128/IAI.72.6.3299-3309.2004

    Figure Lengend Snippet: Probiotic stimulates IL-10 release in DC cultures. BM cells were grown in GM-CSF. Various concentrations of probiotic or bacteria were added on day 2 for a further 3 days. DC culture supernatants were harvested on day 5, and cytokine levels were measured by ELISA. Results are shown for IL-10 (A) and IL-12 (p70) (B). Error bars represent standard deviations from duplicate wells. P values indicate levels of significance above control PBS. Results are representative of three separate experiments.

    Article Snippet: Cytokine detection was done by enzyme-linked immunosorbent assay (ELISA) for interleukin-10 (IL-10) and gamma interferon (IFN-γ) with paired antibodies (PharMingen) and for bioactive IL-12 (p70) with the Duoset DY 419 kit (R&D Systems).

    Techniques: Bacteria, Enzyme-linked Immunosorbent Assay

    Probiotic stimulation of IL-12 (p70). Cells at day 4 of BM DC culture were stimulated for an overnight period by the addition of 107 organisms of probiotic or bacteria/ml until the next day. Supernatants were collected, and IL-12 levels (A) or IL-10 release (B) in supernatants were determined by ELISA. Standard deviation is shown for duplicate wells. The level of significance is indicated by the P value.

    Journal:

    Article Title: Bacterial Probiotic Modulation of Dendritic Cells

    doi: 10.1128/IAI.72.6.3299-3309.2004

    Figure Lengend Snippet: Probiotic stimulation of IL-12 (p70). Cells at day 4 of BM DC culture were stimulated for an overnight period by the addition of 107 organisms of probiotic or bacteria/ml until the next day. Supernatants were collected, and IL-12 levels (A) or IL-10 release (B) in supernatants were determined by ELISA. Standard deviation is shown for duplicate wells. The level of significance is indicated by the P value.

    Article Snippet: Cytokine detection was done by enzyme-linked immunosorbent assay (ELISA) for interleukin-10 (IL-10) and gamma interferon (IFN-γ) with paired antibodies (PharMingen) and for bioactive IL-12 (p70) with the Duoset DY 419 kit (R&D Systems).

    Techniques: Bacteria, Enzyme-linked Immunosorbent Assay, Standard Deviation